Rat Interleukin 12,IL-12/P70 ELISA KIT

產品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠白介素12(IL-12/P70)酶聯(lián)免疫試劑盒（CSB-E07364r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12/P70含量。IL12/P70是重要細胞因子，在免疫調節(jié)中發(fā)揮關鍵作用。它能激活T細胞和NK細胞來增強免疫應答。研究圍繞其信號通路、與疾病關聯(lián)機制展開，旨在通過調節(jié)它的活性開發(fā)治療感染、腫瘤及自身免疫疾病的新療法。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，該產品適用于科研場景中免疫機制研究，如炎癥性疾病模型評估、疫苗免疫效果分析或藥物干預后IL - 12/P70的動態(tài)變化觀測，其中血清/血漿樣本可用于系統(tǒng)性免疫狀態(tài)分析，組織勻漿則適合局部炎癥微環(huán)境研究。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

IL-12/P70
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-106
1:2	Average %	94
	Range %	91-98
1:4	Average %	93
	Range %	89-96
1:8	Average %	87
	Range %	82-96

回收率：

The recovery of rat IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	103	98-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.