Rabbit interleukin 12 (IL-12/P70) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔白介素12(IL-12/P70)酶聯(lián)免疫試劑盒（CSB-EQ027628RB）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL12/P70含量。IL12/P70是重要的免疫靶點(diǎn)。它由p35和p40亞基組成，在調(diào)節(jié)免疫細(xì)胞功能、促進(jìn)Th1細(xì)胞分化等方面發(fā)揮關(guān)鍵作用。其研究機(jī)制主要聚焦于調(diào)控免疫應(yīng)答相關(guān)信號(hào)通路。深入研究它有望為免疫相關(guān)疾病治療提供新策略。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，該產(chǎn)品適用于實(shí)驗(yàn)動(dòng)物模型的免疫調(diào)控研究、感染性疾病機(jī)制探索、疫苗效力評(píng)估及炎癥相關(guān)科研項(xiàng)目，為研究IL-12/P70在免疫微環(huán)境中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

IL12/P70, Interleukin-12/P70
縮寫(xiě)：

IL-12/P70
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-95
1:2	Average %	90
	Range %	83-99
1:4	Average %	95
	Range %	92-102
1:8	Average %	93
	Range %	86-99

回收率：

The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-110
EDTA plasma (n=4)	97	89-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.