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BMI1 Recombinant Monoclonal Antibody

  • 中文名稱：
    BMI1重組抗體
  • 貨號：
    CSB-RA916472A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, U87 whole cell lysate, U251 whole cell lysate
      All lanes: BMI1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 37 kDa
      Observed band size: 45 kDa
    • IHC image of CSB-RA916472A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA916472A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA916472A0HU重組單克隆抗體是針對BMI1蛋白研發(fā)的高特異性科研試劑，經(jīng)嚴(yán)格驗證適用于ELISA、Western Blot及免疫組化等實驗。該抗體靶向BMI1蛋白，該蛋白作為Polycomb抑制復(fù)合體PRC1的核心組分，在表觀遺傳調(diào)控中發(fā)揮關(guān)鍵作用，參與干細胞自我更新、細胞分化及腫瘤發(fā)生等重要生物學(xué)過程。經(jīng)多平臺驗證數(shù)據(jù)顯示，本產(chǎn)品在Western Blot實驗中可清晰識別目標(biāo)蛋白條帶，推薦使用稀釋比例為1:500-1:5000；免疫組化應(yīng)用時能在石蠟包埋組織中準(zhǔn)確定位BMI1的亞細胞分布，推薦工作濃度為1:50-1:200。適用于腫瘤生物學(xué)領(lǐng)域探索癌干細胞特性、腫瘤微環(huán)境調(diào)控等研究，也可用于發(fā)育生物學(xué)中細胞命運決定機制的分析，以及表觀遺傳學(xué)領(lǐng)域內(nèi)染色質(zhì)修飾相關(guān)研究。本產(chǎn)品采用重組表達技術(shù)生產(chǎn)，具有批次間穩(wěn)定性高、背景信號低的特點，可為研究人員提供可靠的實驗數(shù)據(jù)支持。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Polycomb complex protein BMI-1 (Polycomb group RING finger protein 4) (RING finger protein 51), BMI1, PCGF4 RNF51
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human Bmi1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    1F2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1066

    OMIM: 164831

    KEGG: hsa:100532731

    STRING: 9606.ENSP00000365851

    UniGene: Hs.380403