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  4. Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

  • 中文名稱：
    大鼠正常T細(xì)胞表達(dá)和分泌因子/趨化因子5(RANTES/CCL5)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07398r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠正常T細(xì)胞表達(dá)和分泌因子/趨化因子5(RANTES/CCL5)酶聯(lián)免疫試劑盒（CSB-E07398r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的RANTES/CCL5含量。RANTES/CCL5是一種趨化因子，在免疫調(diào)節(jié)、炎癥反應(yīng)等方面有重要背景。其研究機(jī)制涉及多種細(xì)胞，能吸引特定免疫細(xì)胞到炎癥部位，參與免疫應(yīng)答。它與多種疾病如感染、自身免疫病相關(guān)，對它的研究有助于深入了解疾病發(fā)病機(jī)理與治療。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外實(shí)驗(yàn)中對大鼠疾病模型（如關(guān)節(jié)炎、神經(jīng)炎癥或病毒感染模型）中RANTES動態(tài)表達(dá)的定量分析，也可用于藥物研發(fā)過程中評價免疫調(diào)節(jié)劑或抗炎藥物對趨化因子通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
  • 縮寫：
    RANTES/CCL5
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 99
    Range % 94-105
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 92
    Range % 88-96
    1:8 Average % 101
    Range % 97-106
  • 回收率：
    The recovery of rat RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 83-92
    EDTA plasma (n=4) 92 86-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.684 2.799 2.742 2.633
    5 2.178 2.349 2.264 2.155
    2.5 1.444 1.489 1.467 1.358
    1.25 0.783 0.795 0.789 0.680
    0.625 0.376 0.392 0.384 0.275
    0.312 0.258 0.264 0.261 0.152
    0.156 0.201 0.208 0.205 0.096
    0 0.108 0.109 0.109 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數(shù)據(jù)庫鏈接：