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  4. Rat macrophage inflammatory protein 1α,MIP-1α ELISA Kit

Rat macrophage inflammatory protein 1α,MIP-1α ELISA Kit

  • 中文名稱：
    大鼠巨噬細(xì)胞炎癥蛋白1α(MIP-1α/CCL3)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07422r-IS
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠巨噬細(xì)胞炎癥蛋白1α(MIP-1α/CCL3)酶聯(lián)免疫試劑盒（CSB-E07422r-IS）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CCL3含量。CCL3是一個(gè)備受關(guān)注的靶點(diǎn)。它在免疫調(diào)節(jié)等生理過程中發(fā)揮重要作用。相關(guān)研究聚焦其在炎癥、腫瘤等疾病中的機(jī)制，探索其與免疫細(xì)胞招募、活化的關(guān)聯(lián)，試圖通過干預(yù)CCL3來調(diào)控免疫反應(yīng)，為疾病治療提供新策略。試劑盒檢測(cè)范圍為3.9 pg/mL-250 pg/mL，靈敏度為1.95 pg/mL。MIP-1α作為趨化因子家族成員，在炎癥反應(yīng)、免疫細(xì)胞趨化及組織修復(fù)中發(fā)揮關(guān)鍵作用，常用于研究感染性疾病、自身免疫反應(yīng)及損傷修復(fù)等科研場(chǎng)景?？蒲腥藛T可通過該產(chǎn)品定量分析炎癥相關(guān)疾病模型中MIP-1α的動(dòng)態(tài)變化，為探索免疫調(diào)控機(jī)制或評(píng)估抗炎藥物效果提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccl3 ELISA Kit; Mip1a ELISA Kit; Scya3 ELISA Kit; C-C motif chemokine 3 ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; MIP-1-alpha ELISA Kit; Small-inducible cytokine A3 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    3.9 pg/mL-250 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    94

    Range %

    89-99

    1:2

    Average %

    101

    Range %

    94-108

    1:4

    Average %

    97

    Range %

    92-102

    1:8

    Average %

    93

    Range %

    89-98

  • 回收率：

    The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    85-98

    EDTA plasma (n=4)

    97

    90-104

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    250

    2.722

    2.634

    2.678

    2.550

    125

    2.023

    2.087

    2.055

    1.927

    62.5

    1.236

    1.268

    1.252

    1.124

    31.25

    0.765

    0.729

    0.747

    0.619

    15.6

    0.514

    0.501

    0.507

    0.379

    7.8

    0.286

    0.273

    0.280

    0.152

    3.9

    0.225

    0.214

    0.219

    0.091

    0

    0.131

    0.125

    0.128

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
  • 基因功能參考文獻(xiàn)：
    1. The important chemokine macrophage inflammatory protein1alpha increases inflammatory responses and the secretion of inflammatory molecules from Kupffer cells, including tumor necrosis factoralpha, interleukin (IL)1beta and IL6. PMID: 28677732
    2. The present report demonstrates an increase in mRNA and protein levels in chemokines CCL3 and its receptor CCR5 in dorsal root ganglion in the rat model of remifentanil-induced hyperalgesia. Furthermore, the results pointed out that CCL3 and its receptor CCR5 might be implicated in remifentanil-induced hyperalgesia. PMID: 26550961
    3. Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
    4. The results of this study suggest a contribution of CCL3 in the spinal dorsal horn to paclitaxel-induced allodynia and may provide new therapeutic targets for paclitaxel-induced painful neuropathy. PMID: 25127716
    5. Expression levels of CCL3 in the spinal cord are up-regulated following nerve injury. PMID: 24589480
    6. NMDA-evoked neuronal injury differentially induces MIP-1alpha and MCP-1 production in microglia and astrocytes, respectively, through activation of different intracellular signaling pathways. PMID: 22791363
    7. Our data suggest that free radicals produced in CA1 neurons contribute to the recruitment and activation of microglia and neurodegeneration through MIP-1alpha expression. PMID: 18272692
    8. In this study we present data on long term increase in CCL3 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
    9. Data show significant concentration increases for CXCL1 and MIP-1alpha in the brain after soman-induced seizure onset. PMID: 21535896
    10. The active behavior of the MIP-1alpha gene in KCs following liver ischemia/reperfusion injury is assumed to be one of the major causes for the hepatic ischemia/reperfusion injury. PMID: 16804970
    11. P2Y6 activation by uridine 5'-diphosphate induces Ccl2 and Ccl3 expression in primary cultured microglia, astrocytes, and brain slices from neonatal and adult rats. PMID: 21317391
    12. a massive amount of ATP molecules released in the glioma tumor site may act as the regulator with P2XR signaling that increases MIP-1alpha and MCP-1 expression in tumor-infiltrating microglia/macrophages. PMID: 21162127
    13. These results suggest that MIP-1alpha is directly involved in the neurodegeneration induced in rats by PVC-211 murine leukemia virus by recruiting macrophages/microglia from the periphery into regions of the brain that eventually become diseased. PMID: 21051067
    14. expression is regulated by nicotine in alveolar macrophages PMID: 12101081
    15. The constitutive patterns of MIP-1 alpha mRNA and protein expression are demonstrated in rat brain throughout postnatal development within local microenvironments where the postnatal cell migration phenomenon occurs. PMID: 12899200
    16. Oligodendroglia express the CCL3 receptor CCR1, but not CCR5. CCL3 induced an intracellular rise in Ca(2+), a typical signalling event of G protein-coupled receptors. PMID: 14583384

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數(shù)據(jù)庫鏈接：