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  2. 試劑盒
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  4. Rat TNF-α ELISA kit
  • 中文名稱(chēng)：
    大鼠腫瘤壞死因子α（TNF-α）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11987r-IS
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠腫瘤壞死因子α（TNF-α）酶聯(lián)免疫試劑盒（CSB-E11987r-IS）為雙抗夾心法ELISA試劑盒，定量檢測(cè)組織勻漿、組織培養(yǎng)上清液、支氣管肺泡灌洗液樣本中的TNF含量。TNF（腫瘤壞死因子）是一種重要的細(xì)胞因子，主要由活化的巨噬細(xì)胞產(chǎn)生，具有抑制腫瘤細(xì)胞增殖、調(diào)節(jié)免疫細(xì)胞功能等作用。其研究機(jī)制涉及與TNFR1和TNFR2受體結(jié)合，激活下游信號(hào)通路，影響細(xì)胞存活、凋亡和炎癥反應(yīng)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于組織勻漿、細(xì)胞培養(yǎng)上清及支氣管肺泡灌洗液樣本分析；可滿(mǎn)足炎癥機(jī)制研究、藥物干預(yù)效果評(píng)估、病理模型建立等科研需求，特別適用于敗血癥、關(guān)節(jié)炎、腦損傷等動(dòng)物模型中炎癥因子的動(dòng)態(tài)監(jiān)測(cè)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    tissue homogenates, cell culture supernates, bronchoalveolar lavage fluid
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    103

    Range %

    98-108

    1:2

    Average %

    95

    Range %

    88-101

    1:4

    Average %

    98

    Range %

    92-105

    1:8

    Average %

    92

    Range %

    86-98

  • 回收率：

    The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    89-100

    EDTA plasma (n=4)

    87

    82-93

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.717

    2.634

    2.675

    2.487

    500

    1.476

    1.467

    1.471

    1.283

    250

    0.853

    0.908

    0.881

    0.693

    125

    0.608

    0.629

    0.619

    0.431

    62.5

    0.395

    0.381

    0.388

    0.200

    31.2

    0.281

    0.293

    0.287

    0.099

    15.6

    0.218

    0.207

    0.213

    0.025

    0

    0.191

    0.185

    0.188

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 數(shù)據(jù)庫(kù)鏈接：