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  4. Rat Fibronectin,FN ELISA Kit

Rat Fibronectin,FN ELISA Kit

  • 中文名稱：
    大鼠纖連蛋白(FN)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04553r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠纖連蛋白(FN)酶聯(lián)免疫試劑盒（CSB-E04553r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的FN1含量。FN1，即纖連蛋白1，是一種存在于血漿和細(xì)胞外基質(zhì)中的糖蛋白，參與細(xì)胞粘附和遷移等過程。研究顯示，F(xiàn)N1在運(yùn)動(dòng)中通過激活肝臟自噬和改善胰島素敏感性來促進(jìn)機(jī)體健康，其具體機(jī)制涉及骨骼肌分泌FN1與肝臟α5β1整合素受體結(jié)合，進(jìn)而調(diào)節(jié)肝臟自噬和胰島素敏感性。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，適用于體外培養(yǎng)細(xì)胞分泌功能評(píng)估、組織工程模型基質(zhì)成分分析，以及心血管疾病、纖維化疾病等動(dòng)物模型中細(xì)胞外基質(zhì)動(dòng)態(tài)變化的科研探索，助力于細(xì)胞行為機(jī)制、病理生理過程及相關(guān)藥物篩選等基礎(chǔ)研究領(lǐng)域的數(shù)據(jù)獲取。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Fn1Fibronectin ELISA kit; FN) [Cleaved into: Anastellin] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %96
    Range %85-100
    1:2000Average %87
    Range %82-93
    1:4000Average %98
    Range %95-100
    1:8000Average %92
    Range %88-94
  • 回收率：
    The recovery of rat FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)9989-109
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.370 2.405 2.388 2.245
    252.015 2.063 2.039 1.896
    12.51.613 1.627 1.620 1.477
    6.251.115 1.136 1.126 0.983
    3.120.685 0.679 0.682 0.539
    1.560.432 0.467 0.450 0.307
    0.780.278 0.291 0.285 0.142
    00.142 0.144 0.143 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
  • 數(shù)據(jù)庫(kù)鏈接：