Rat Collagen Type Ⅳ,Col Ⅳ ELISA KIT
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中文名稱(chēng):大鼠Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08883r
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規(guī)格:96T/48T
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價(jià)格:¥3900/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒(CSB-E08883r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的Col Ⅳ含量。Col Ⅳ即Ⅳ型膠原,是基底膜的主要成分。它在維持組織正常結(jié)構(gòu)與功能中發(fā)揮重要作用。研究發(fā)現(xiàn)其異常表達(dá)與腫瘤侵襲、轉(zhuǎn)移及多種疾病相關(guān),機(jī)制可能涉及與細(xì)胞表面受體相互作用,影響細(xì)胞黏附、遷移和信號(hào)傳導(dǎo)等過(guò)程。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL,可應(yīng)用于肝/腎纖維化動(dòng)物模型構(gòu)建、血管基底膜損傷研究、腫瘤微環(huán)境分析等科研領(lǐng)域,特別適用于評(píng)估疾病模型中Col Ⅳ的動(dòng)態(tài)變化及組織損傷修復(fù)機(jī)制,為心血管疾病、器官纖維化等病理過(guò)程的分子機(jī)制研究提供精準(zhǔn)檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
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縮寫(xiě):Col Ⅳ
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種屬:Rattus norvegicus (Rat)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.39 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-105 1:2 Average % 96 Range % 92-101 1:4 Average % 92 Range % 87-96 1:8 Average % 87 Range % 82-94 -
回收率:
The recovery of rat COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 104 99-108 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.221 2.120 2.171 2.019 50 1.441 1.340 1.391 1.239 25 0.825 0.804 0.815 0.663 12.5 0.519 0.530 0.525 0.373 6.25 0.335 0.324 0.330 0.178 3.12 0.240 0.251 0.246 0.094 1.56 0.196 0.192 0.194 0.042 0 0.152 0.151 0.152 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Decellularized Rat Tubules for tissue engineering Q Jia, K Li, T Liu, F Kong, S Zhao, X Yang, Y Guan,research square,2025
- Effects of novel Fufang Biejia Ruangan Tablets with sheep placenta as substitute for Hominis Placenta on CCl4-induced liver fibrosis B Shen,Chinese Herbal Medicines,2023
- mTOR/ULK1 signaling axis mediated ultrashort wave regulation of autophagy to alleviate diabetic kidney disease Y Yan,Advances in clinical and experimental medicine,2023
- Oxymatrine exerts anti-fibrotic effects in a rat model of hepatic fibrosis by suppressing endoplasmic reticulum stress X Liu,Journal of International Medical Research,2020
- Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats M Al Za'abi,Naunyn-Schmiedeberg's Archives of Pharmacology,2020
- Collagen IV, a promising serum biomarker for evaluating the prognosis of revascularization in a 2-kidney, 1-clip hypertensive rat model Liqiang Li, et al,Interactive Cardiovascular and Thoracic Surgery,2019
- Role of betaine in liver injury induced by the exposure to ionizing radiation Shedid SM.et al,environmental toxicology,2018
- Hepatoprotective effects of Dicliptera chinensis polysaccharides on dimethylnitrosamine-induced hepatic fibrosis rats and its underlying mechanism Zhang K. et al,J Ethnopharmacol,2015
- Counteraction between angiotensin II and angiotensin-(1-7) via activating angiotensin type I and Mas receptor on rat renal mesangial cells Hong Xue et al,Regulatory Peptides,2012
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