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  4. Rabbit Interleukin 8,IL-8 ELISA Kit

Rabbit Interleukin 8,IL-8 ELISA Kit

  • 中文名稱：
    兔子白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06905Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒（CSB-E06905Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8是一種趨化因子，在炎癥反應(yīng)、免疫調(diào)節(jié)等生理病理過程中發(fā)揮重要作用。其研究機(jī)制主要聚焦于與受體結(jié)合，介導(dǎo)細(xì)胞趨化和激活，可調(diào)節(jié)中性粒細(xì)胞遷移和活化，在感染、腫瘤、自身免疫病等疾病中表達(dá)常異常，是潛在治療靶點(diǎn)。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，適用于實(shí)驗(yàn)室中炎癥機(jī)制研究、免疫調(diào)控實(shí)驗(yàn)及藥物開發(fā)過程中的生物標(biāo)志物動(dòng)態(tài)監(jiān)測，例如在細(xì)菌感染模型、創(chuàng)傷修復(fù)研究或抗炎藥物藥效評估中量化IL - 8的表達(dá)變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant/activation protein 1 ELISA Kit; NAP-1 ELISA Kit; Permeability factor 1 ELISA Kit; PF1 ELISA Kit; rPF1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %101
    Range %94-105
    1:2Average %97
    Range %91-102
    1:4Average %89
    Range %85-94
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of rabbit IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-96
    EDTA plasma (n=4)9289-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.390 2.345 2.368 2.285
    7501.587 1.476 1.532 1.449
    3750.814 0.778 0.796 0.713
    187.50.409 0.389 0.399 0.316
    940.276 0.264 0.270 0.187
    470.182 0.172 0.177 0.094
    23.50.134 0.131 0.133 0.050
    00.085 0.081 0.083 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

平均分:
5.0分 - 1 個(gè)評價(jià)

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他 兔

稀釋比：沒有稀釋

產(chǎn)品評價(jià)： 我用CSB-E06905Rb檢測模型組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來的R2為0.9998，見圖片，OD值范圍為0.04（Blank)-2.5(1500pg/ml)，標(biāo)準(zhǔn)曲線符合預(yù)期。部分樣本低于標(biāo)曲的Blank吸光度，經(jīng)過文獻(xiàn)查詢，該模型需要6h才能檢測出大量IL-8炎癥因子水平，因此屬于正?，F(xiàn)象。且得到的數(shù)據(jù)符合預(yù)期趨勢，后續(xù)會(huì)測量6h及以上的炎癥因子水平。另回答郵件中的疑問：我做的是術(shù)前，術(shù)中0-2h，術(shù)后24h共6個(gè)點(diǎn)的指標(biāo)，沒做6h的指標(biāo)，因?yàn)?h兔子耳朵采不出動(dòng)脈血了，所以后續(xù)會(huì)在6h處死兔子來檢測炎癥指標(biāo)。

By 陳老師

靶點(diǎn)詳情

  • 功能：
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
  • 基因功能參考文獻(xiàn)：
    1. At the 2-wk time point, interleukin 8 gene expression was comparable in intervertebral discs injected with chondrocytes in controls, whereas its expression in IVDs injected with saline increased 50-fold PMID: 25133623
    2. Data show that the serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) decreased significantly in Dachengqi decoction treatment group. PMID: 25270202
    3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
    4. IL-8 and IL-1ra showed correlation with the severity of colitis. These observations should significantly further understandings on the role of neutrophils and monocytes in the immunopathogenesis of ulcerative colitis PMID: 21807533
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 數(shù)據(jù)庫鏈接：