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  4. Rabbit Interleukin 10,IL-10 ELISA Kit

Rabbit Interleukin 10,IL-10 ELISA Kit

  • 中文名稱：
    兔子白介素10(IL-10)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06897Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子白介素10(IL-10)酶聯(lián)免疫試劑盒（CSB-E06897Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL10含量。IL10是一種重要的免疫調(diào)節(jié)細(xì)胞因子。它可抑制多種促炎細(xì)胞因子的產(chǎn)生，從而發(fā)揮抗炎作用。目前關(guān)于IL10的研究聚焦于其在自身免疫病、感染性疾病等疾病中的作用機(jī)制，有望為相關(guān)疾病的治療提供新靶點(diǎn)和新思路。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品廣泛應(yīng)用于免疫學(xué)研究領(lǐng)域，特別適用于評(píng)估炎癥性疾病模型、感染免疫應(yīng)答或免疫調(diào)節(jié)藥物干預(yù)實(shí)驗(yàn)中IL-10的動(dòng)態(tài)變化，為探索免疫調(diào)控機(jī)制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-98
    1:2Average %96
    Range %91-105
    1:4Average %97
    Range %92-110
    1:8Average %93
    Range %86-100
  • 回收率：
    The recovery of rabbit IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)8980-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.627 2.549 2.588 2.461
    10001.954 1.799 1.877 1.750
    5001.004 0.987 0.996 0.869
    2500.599 0.554 0.577 0.450
    1250.369 0.361 0.365 0.238
    62.50.274 0.264 0.269 0.142
    31.250.177 0.171 0.174 0.047
    00.132 0.122 0.127 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 數(shù)據(jù)庫鏈接：