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  4. Pig Interleukin 15(IL-15) ELISA Kit

Pig Interleukin 15(IL-15) ELISA Kit

  • 中文名稱：
    豬白介素15(IL-15)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14320p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素15(IL-15)酶聯(lián)免疫試劑盒（CSB-E14320p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的IL15含量。IL-15是一種細(xì)胞因子，在免疫系統(tǒng)調(diào)節(jié)中至關(guān)重要。它能促進(jìn)T細(xì)胞和NK細(xì)胞的存活、增殖及活化，增強機體免疫功能。目前關(guān)于它的研究聚焦于其在腫瘤免疫治療、自身免疫病等方面的機制，為相關(guān)疾病提供新的治療思路。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，該產(chǎn)品廣泛應(yīng)用于動物免疫學(xué)、疾病機制研究及疫苗開發(fā)等領(lǐng)域，例如用于評估豬群免疫狀態(tài)、研究病原體感染過程中細(xì)胞因子的動態(tài)變化，或分析體外免疫細(xì)胞培養(yǎng)模型中IL - 15的分泌水平，為科研人員探索豬類免疫調(diào)控網(wǎng)絡(luò)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測范圍：
    0.78 pg/mL-50 pg/mL
  • 靈敏度：
    0.195 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %80-89
    1:2Average %102
    Range %97-107
    1:4Average %87
    Range %84-91
    1:8Average %84
    Range %81-89
  • 回收率：
    The recovery of pig IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10097-103
    EDTA plasma (n=4)8480-88
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    502.602 2.576 2.589 2.400
    251.879 1.850 1.865 1.676
    12.51.144 1.168 1.156 0.967
    6.250.683 0.691 0.687 0.498
    3.120.452 0.468 0.460 0.271
    1.560.361 0.391 0.376 0.187
    0.780.288 0.298 0.293 0.104
    00.191 0.186 0.189
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.
  • 基因功能參考文獻(xiàn)：
    1. Study identified two completely linked SNPs in the porcine IL15 promoter region that could alter IL15 transcription activity. As interleukin-15 can inhibit porcine adipocyte differentiation, these promoter mutations could affect intramuscular fat deposition by producing differential levels of muscle-derived interleukin-15. PMID: 29168191
    2. Myokine IL-15 regulates the crosstalk of co-cultured porcine skeletal muscle satellite cells and preadipocytes. PMID: 25098601
    3. results demonstrate that porcine reproductive and respiratory syndrome virus (PRRSV)infection could induce IL-15 production in macrophages/dendritic cells; data further show that upregulation of IL-15 by PRRSV requires PKC and NF-kappaB pathways PMID: 22573868
    4. IFN-gamma targets the adipocyte to induce IL-15 expression, thus indicating a possible role for the adipocyte in the regulation of T-cell function and muscle metabolism during the innate immune response PMID: 14656772
    5. When induced by IFN-gamma or other inflammatory mediators, IL-15 may be a significant homeorhetic factor that mobilizes and directs energy away from the adipocyte to other cells during the acute phase of the inflammatory response. PMID: 15155279

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-15/IL-21 family
  • 數(shù)據(jù)庫鏈接：