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  4. Mouse Vascular Endothelial cell Growth Factor B,VEGF-B ELISA kit

Mouse Vascular Endothelial cell Growth Factor B,VEGF-B ELISA kit

  • 中文名稱：
    小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子B(VEGF-B)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07365m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子B(VEGF-B)酶聯(lián)免疫試劑盒（CSB-E07365m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的VEGFB含量。VEGFB即血管內(nèi)皮生長(zhǎng)因子B，是VEGF家族成員。其廣泛表達(dá)于心臟、骨骼肌等組織。研究機(jī)制上，VEGFB主要通過與受體結(jié)合，調(diào)控血管內(nèi)皮細(xì)胞等的生長(zhǎng)、存活與遷移，在血管生成等生理病理過程中發(fā)揮重要作用，為相關(guān)疾病治療提供潛在靶點(diǎn)。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，本試劑盒適用于體外培養(yǎng)細(xì)胞模型、動(dòng)物組織樣本及體液中的VEGF - B水平分析，可滿足心血管疾病模型構(gòu)建、血管生成機(jī)制研究及代謝相關(guān)基礎(chǔ)實(shí)驗(yàn)的定量檢測(cè)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Vegfb ELISA kit; VrfVascular endothelial growth factor B ELISA kit; VEGF-B ELISA kit; VEGF-related factor ELISA kit; VRF ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    0.195 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %92-102
    1:2Average %91
    Range %84-97
    1:4Average %90
    Range %87-94
    1:8Average %100
    Range %95-103
  • 回收率：
    The recovery of mouse VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-93
    EDTA plasma (n=4)9390-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.743 2.763 2.753 2.589
    2502.405 2.435 2.420 2.256
    1251.756 1.796 1.776 1.612
    62.50.978 1.016 0.997 0.833
    31.20.501 0.535 0.518 0.354
    15.60.351 0.359 0.355 0.191
    0.780.237 0.255 0.246 0.082
    00.162 0.165 0.164 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults.
  • 基因功能參考文獻(xiàn)：
    1. VEGF-B is a potent regulator of the antioxidant pathway in retina.The antioxidant function of VEGF-B is mediated mainly by VEGFR1. PMID: 30249667
    2. the expression and role of VEGF-B in diabetic corneal neuropathy was examined by using type 1 diabetic mice and cultured trigeminal ganglion (TG) neurons. PMID: 28091556
    3. Data suggest that dietary fatty acids create epigenetic/nutrigenomic changes in methylation levels of CpG island at Vegfb promoter and changes in Vegfb expression in white adipocytes in vivo and in vitro, with particular up-regulation of DNA methylation by linoleic acid; Vegfb promoter methylation levels are closely related to Vegfb gene expression in visceral and subcutaneous adipose tissue. PMID: 26707994
    4. renal VEGF-B expression correlates with the severity of Diabetic Kidney Disease. PMID: 28190774
    5. PGC-1alpha is a master metabolic sensor that regulates the expression of Vegfa and Vegfb, coordinates blood vessels growth and fatty acid uptake with mitochondrial fatty acid oxidation. PMID: 26822083
    6. These data therefore support a tightly controlled, paracrine signaling mechanism of VEGF-B to VEGFR1. PMID: 26928042
    7. VEGFB-Induced Vascular Remodeling in Adipose Tissue Requires VEGF/VEGFR2. VEGFB-Induced Vascular Remodeling Improves Insulin Supply, Signaling, and Function in Obese Mice. PMID: 27076080
    8. VEGF-B is dispensable for normal cardiac function under unstressed conditions and for high fat diet-induced metabolic changes. PMID: 25168313
    9. VEGF-B is a vascular remodeling factor promoting cancer metastasis. PMID: 25991856
    10. Data indicate that vascular endothelial growth factor B knockout (Vegf-b-/-) mice showed impaired nerve repair with concomitant impaired trophic function. PMID: 25404333
    11. Data indicate that VEGF-B is a high-affinity VEGFR-1 ligand that, unlike PlGF, cannot efficiently induce signaling downstream of VEGFR-1. PMID: 23821770
    12. results demonstrate that the vascular endothelium can function as an efficient barrier to excess muscle lipid uptake even under conditions of severe obesity and type 2 diabetes, and that this barrier can be maintained by inhibition of VEGF-B signalling PMID: 23023133
    13. the RTEF-1-driven increase of VEGF-B plays an important role in communication between the endothelium and myocardium PMID: 21169295
    14. Study indicates that VEGF-B, instead of acting as an angiogenic factor, exerts direct neuroprotective effects through FLT1. PMID: 21248239
    15. Data show that no differences in vascular density, perfusion or immune cell infiltration upon altered Vegfb gene dosage were noted. PMID: 21124841
    16. VEGF-B appears to be a coronary growth factor in rats but not in mice. PMID: 20937974
    17. VEGF-B is involved in the retinal recovery processes and plays a potent role of neuroprotection in retinal ganglion cells after the optic nerve crush. PMID: 19484929
    18. VEGF-B specifically controlled endothelial uptake of fatty acids via transcriptional regulation of vascular fatty acid transport proteins PMID: 20228789
    19. Assignment of the mouse Vegfb gene to mouse chromosome 19 B PMID: 12063409
    20. when overexpressed in the lung, VEGF-B can be as potent as VEGF-A in attenuating pulmonary hypertension, although it has no effect on eNOS expression or vascular permeability. PMID: 12547729
    21. VEGF-B, in part through its receptor VEGF-R1, promotes angiogenesis in association with an activation of Akt and eNOS-related pathways PMID: 12805240
    22. The reduction in inflammation-associated synovial angiogenesis in Vegfb(-/-) mice implicates VEGF-B in pathologic vascular remodeling in inflammatory arthritis. PMID: 13130487
    23. endogenous VegfB may act to protect the brain from ischemic injury PMID: 15529014
    24. a direct effect of VEGF-B isoforms in potentiating angiogenesis. PMID: 16109918
    25. These results suggest a role for VEGFB in the regulation of adult neurogenesis, which could have therapeutic implications for diseases associated with central neuronal loss PMID: 16337622
    26. the role of endogenous antiangiogenic VEGF isoforms in normal physiology--their down-regulation is required for effective milk production PMID: 18032632
    27. VEGF-B appears to have a relatively restricted angiogenic activity in the ischemic heart. PMID: 18511699
    28. The 60 kDa VEGF-B(186) isoform is a neuroprotective factor that protects primary motor neurons against degeneration. PMID: 18923022
    29. function of VEGF-B in the vascular system is to act as a "survival," rather than an "angiogenic" factor and that VEGF-B inhibition may offer new therapeutic opportunities to treat neovascular diseases PMID: 19369214

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  • 亞細(xì)胞定位：
    Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 組織特異性：
    Abundantly expressed in heart, brain, kidney and skeletal muscle.
  • 數(shù)據(jù)庫鏈接：