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  4. Mouse Mannan-binding lectin serine protease 2(MASP2) ELISA kit

Mouse Mannan-binding lectin serine protease 2(MASP2) ELISA kit

  • 中文名稱：
    小鼠甘露糖結(jié)合凝集素相關(guān)絲氨酸蛋白酶2(MASP2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL013510MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠甘露糖結(jié)合凝集素相關(guān)絲氨酸蛋白酶2(MASP2)酶聯(lián)免疫試劑盒（CSB-EL013510MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MASP2含量。MASP2是一種血清蛋白酶，通過甘露糖結(jié)合凝集素激活補體系統(tǒng)，裂解C2和C4，形成C3轉(zhuǎn)化酶，是補體級聯(lián)中的關(guān)鍵步驟。其研究機制涉及MASP2的激活、裂解和補體系統(tǒng)的激活過程。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于免疫學(xué)、炎癥機制、補體系統(tǒng)功能等領(lǐng)域的基礎(chǔ)研究，如探索感染性疾病模型、自身免疫疾病動物實驗中MASP2的動態(tài)變化及其調(diào)控機制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Masp2Mannan-binding lectin serine protease 2 ELISA Kit; EC 3.4.21.104 ELISA Kit; MBL-associated serine protease 2 ELISA Kit; Mannose-binding protein-associated serine protease 2 ELISA Kit; MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain; Mannan-binding lectin serine protease 2 B chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.2 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MASP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 92    
    Range % 89-95    
    1:200 Average % 101    
    Range % 97-106    
    1:400 Average % 86    
    Range % 83-90    
    1:800 Average % 102    
    Range % 97-107    
  • 回收率：
    The recovery of mouse MASP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-93    
    EDTA plasma (n=4) 100 95-105    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average      
    8000 0.100 0.106 0.103      
    4000 0.243 0.240 0.242      
    2000 0.358 0.361 0.360      
    1000 0.516 0.531 0.524      
    500 0.701 0.740 0.721      
    250 1.027 1.100 1.064      
    125 1.507 1.496 1.502      
    0 2.311 2.298 2.305      
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.
  • 基因功能參考文獻：
    1. MASP-2 has an essential role in the mediation of cerebral ischemia-reperfusion injury. PMID: 27577570
    2. this study shows that MASP-2 contributes to the development of collagen Ab-induced arthritis PMID: 28739878
    3. We induced anti-myeloperoxidase vasculitis in mice and showed that neither MASP-2- nor properdin-deficient mice were protected, suggesting that alternative pathway activation does not require properdin or the lectin pathway. PMID: 27235854
    4. Mannan-binding lectin-associated serine protease 2 is critical for the development of renal ischemia reperfusion injury and mediates tissue injury in the absence of complement C4. PMID: 24868011
    5. Gene targeting of mouse TDP-43 negatively affects Masp2 expression. PMID: 24740308
    6. ALI in H5N1-infected mice was caused by excessive complement activation, as demonstrated by deposition of C3, C5b-9, and MBL-C in lung tissue, and by up-regulation of MBL-associated serine protease-2 and the complement receptors C3aR and C5aR. PMID: 23526211
    7. study corroborates the essential function of MASP-2 in the lectin pathway and highlights the importance of mannan-binding lectin-independent lectin pathway activation in the host defense against pneumococci PMID: 22792067
    8. Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury. PMID: 21502512
    9. Highly conserved intron sequences. Restricted expression contrasts with the ubiquitous expression of neighboring genes. PMID: 15672593
    10. Recombinant protein of masp2 was made and purified. PMID: 15826378
    11. MASP-2 is essential for the activation of C4 and sMAP plays a regulatory role in the activation of the lectin pathway. PMID: 17142762

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Plasma.
  • 數(shù)據(jù)庫鏈接：