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  4. Mouse Interferon epsilon(IFNE) ELISA kit

Mouse Interferon epsilon(IFNE) ELISA kit

  • 中文名稱：
    小鼠干擾素ε(IFNE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011049MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠干擾素ε(IFNE)酶聯(lián)免疫試劑盒（CSB-EL011049MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNE含量。IFNE 即Ⅰ型干擾素，它在固有免疫和適應(yīng)性免疫中有關(guān)鍵作用。研究發(fā)現(xiàn)，IFNE 能調(diào)節(jié)免疫細(xì)胞活性、增強(qiáng)抗病毒能力，還可參與腫瘤免疫監(jiān)視。其作用機(jī)制包括激活相關(guān)信號通路，誘導(dǎo)抗病毒蛋白產(chǎn)生，抑制病毒復(fù)制與腫瘤細(xì)胞生長。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎(chǔ)科研中探究IFN - ε在生殖生物學(xué)、免疫調(diào)控機(jī)制或感染性疾病模型中的作用，如卵巢/子宮組織樣本分析、妊娠相關(guān)體液因子檢測或黏膜免疫系統(tǒng)功能研究。數(shù)據(jù)結(jié)果可用于分子機(jī)制研究或生物標(biāo)志物篩選，但不適用于工業(yè)生產(chǎn)或藥物開發(fā)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ifne ELISA Kit; Ifne1 ELISA Kit; Ifnt1Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit; Interferon tau-1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 83-88
    1:2 Average % 94
    Range % 89-98
    1:4 Average % 103
    Range % 97-108
    1:8 Average % 99
    Range % 95-104
  • 回收率：
    The recovery of mouse IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-101
    EDTA plasma (n=4) 89 83-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.271 2.366 2.319 2.143
    500 1.563 1.625 1.594 1.418
    250 0.944 0.956 0.950 0.774
    125 0.582 0.581 0.582 0.406
    62.5 0.382 0.377 0.380 0.204
    31.2 0.274 0.289 0.282 0.106
    15.6 0.220 0.235 0.228 0.052
    0 0.172 0.179 0.176 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral, including HSV-2, and bacterial, including Chlamydia muridarum, genital infections.
  • 基因功能參考文獻(xiàn)：
    1. IFN-epsilon is constitutively expressed by cells of the reproductive tract and is inefficiently secreted by fibroblasts and cell lines. PMID: 23951133
    2. IFN-epsilon could promote migration of antigen-specific CD8(+)T cells to the gut. PMID: 22617838
    3. Ifn-epsilon-deficient mice had increased susceptibility to infection of the female reproductive tract by the common sexually transmitted infections (STIs) herpes simplex virus 2 and Chlamydia muridarum. PMID: 23449591
    4. interactions between estradiol and IFN-tau in the context of the overall immune response during autoimmune orchitis PMID: 21787220
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 組織特異性：
    Expressed at very high levels in uterus and, at much lower levels, in ovary and cervix. Very low levels, if any, in other organs. In the endometrium, expressed in the luminal and glandular epithelial cells (at protein level).
  • 數(shù)據(jù)庫鏈接：