Mouse Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit
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中文名稱:小鼠粒細(xì)胞集落刺激因子(G-CSF)酶聯(lián)免疫試劑盒
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貨號:CSB-E04564m
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規(guī)格:96T/48T
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價(jià)格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠粒細(xì)胞集落刺激因子(G-CSF)酶聯(lián)免疫試劑盒(CSB-E04564m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿,組織勻漿樣本中的CSF3含量。CSF3,即集落刺激因子3,是IL-6超家族的一員,主要在骨髓造血細(xì)胞中表達(dá),通過與其特異性受體結(jié)合,激活JAK/STAT、MAPK等信號通路,促進(jìn)粒細(xì)胞增殖、分化和存活,參與免疫系統(tǒng)發(fā)育和功能,尤其對中性粒細(xì)胞生成和活化有重要作用。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL,靈敏度為1.95 pg/mL。該產(chǎn)品可廣泛應(yīng)用于免疫學(xué)、炎癥機(jī)制研究或藥物開發(fā)等領(lǐng)域,例如評估實(shí)驗(yàn)動(dòng)物模型中G-CSF的動(dòng)態(tài)變化、研究炎癥性疾病相關(guān)通路或篩選調(diào)節(jié)中性粒細(xì)胞生成的化合物,為科研人員探索G-CSF在生理調(diào)控及病理狀態(tài)下的功能提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Csf3 ELISA kit; CsfgGranulocyte colony-stimulating factor ELISA kit; G-CSF ELISA kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma,tissue homogenates
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檢測范圍:7.8 pg/mL-500 pg/mL
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靈敏度:1.95 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
62.693
62.801
62.477
62.585
62.155
62.908
SD
0.033
0.028
0.030
0.037
0.042
0.039
CV(%)
4.342
3.679
3.958
4.875
5.563
5.118
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
87
Range %
85-89
1:2
Average %
102
Range %
99-106
1:4
Average %
98
Range %
96-102
1:8
Average %
92
Range %
90-94
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回收率:
The recovery of mouse G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
89
85-93
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
500
2.897
2.964
2.931
2.785
250
2.089
2.103
2.096
1.950
125
1.311
1.330
1.321
1.175
62.5
0.724
0.711
0.718
0.572
31.2
0.479
0.454
0.467
0.321
15.6
0.319
0.342
0.331
0.185
7.8
0.234
0.244
0.239
0.093
0
0.141
0.151
0.146
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- The construction and immunogenicity analyses of a recombinant pseudorabies virus with porcine circovirus type 3 capsid protein co-expression L Yao,Veterinary microbiology,2023
- Gut microbiota mediated the therapeutic efficiency of Simiao decoction in the treatment of gout arthritis mice X Lin,BMC complementary medicine and therapies,2023
- The Inflammatory Factors Associated with Disease Severity to Predict COVID-19 Progression W Huang,The Journal of Immunology,2021
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:CSF3(集落刺激因子3),也稱為粒細(xì)胞集落刺激因子(G-CSF),是一種細(xì)胞因子,主要由活化的巨噬細(xì)胞、肺泡巨噬細(xì)胞、成纖維細(xì)胞和內(nèi)皮細(xì)胞等產(chǎn)生。它在免疫系統(tǒng)中發(fā)揮著促進(jìn)粒細(xì)胞分化、增殖和釋放的重要作用,對于粒細(xì)胞的生成和免疫應(yīng)答有著重要的調(diào)節(jié)作用。目前,CSF3已被廣泛應(yīng)用于臨床上,例如在化療后恢復(fù)骨髓造血功能、移植后恢復(fù)造血功能以及血液疾病等方面。此外,也有研究表明,CSF3在一些自身免疫性疾病和腫瘤中可能起著重要的作用。
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功能:Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
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基因功能參考文獻(xiàn):
- We confirmed that acute lung injury was associated with high serum G-CSF levels, and elevated neutrophil elastase activity in the lungs and serum of mice with adenine-induced acute kidney injury (AKI). G-CSF could be a target for new anti-lung injury strategy in patients with AKI. PMID: 30056257
- The opposing roles of G-CSF and IFNgamma in regulation of innate inflammatory responses in a murine viral encephalitis model reveal G-CSF as a potential therapeutic target. PMID: 29352287
- central regulator of the transition to postinflammatory chronic visceral pain PMID: 28973941
- We propose that in aggressive pancreatic ductal adenocarcinoma , elevated G-CSF contributes to tumor progression through promoting increases in infiltration of neutrophil-like cells with high immunosuppressive activity. Such a mechanism provides an avenue for a neoadjuvant therapeutic approach for this devastating disease PMID: 28775207
- these data provide strong evidence for a role for G-CSF in the development of ACI after burn injury through suppression of EPO signaling in bone marrow erythroid cells. PMID: 28867537
- physiologically produced G-CSF not only acts as a neutrophil mobilizer at the relatively late stage of acute inflammation, but also prevents exaggerated neutrophil mobilization. PMID: 27551153
- data demonstrate that G-CSF is a pivotal driver of the disease progression in the K/BxN serum-transfer arthritis (STA) model and possibly acts in part by regulating neutrophil numbers in the circulation PMID: 26848119
- SB203580 increases G-CSF expression in macrophages by increasing the stability of G-CSF mRNA via its 3'UTR, and the effect was not due to its inhibition of p38 MAPK activity. PMID: 26772539
- Results suggested that G-CSF plays an important role in preventing colitis, likely through populating immune regulatory macrophages in the intestine. PMID: 26687628
- findings provide convincing evidence that monophosphoryl lipid A-induced G-CSF facilitates early expansion, mobilization, and recruitment of neutrophils to the site of infection after burn injury PMID: 26538529
- Overexpression of VEGF may compensate for the G-CSF deficit through preservation of cellular components, including blood vessels, in the postinfarction heart. PMID: 25976246
- rapidly induces autophagy after spinal cord injury to inhibit neuronal apoptosis PMID: 26524416
- G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy. PMID: 25865621
- constitutive activation of the NF-kappaB pathway in CAIX-depleted cells restored G-CSF secretion PMID: 25623234
- Exposure of Sca-1(+) cells to G-CSF in the culture medium for 72 h induced time-dependent but self-limiting cell cycle acceleration with a restricted effect on the CSC proliferation. PMID: 25160782
- these results suggest that the G-CSF pathway regulates the production of autoantibodies in murine models of lupus. PMID: 23566364
- G-CSF acts in a cell intrinsic manner to expand multipotent progenitors to increase production of tumor-derived Ly6G+ neutrophils. PMID: 25624500
- Endothelial cell(EC)-intrinsic MYD88 signaling and subsequent G-CSF production by ECs is required for myeloid progenitor lineage skewing toward granulocyte-macrophage progenitors, increased colony-forming unit granulocyte activity. PMID: 24990886
- Adventitial CXCL1/granulocyte-colony stimulating factor expression in response to aortic dissection triggers local neutrophil recruitment and activation. This leads to adventitial inflammation via IL-6 and results in aortic expansion and rupture. PMID: 25563839
- Cultured mouse enteric nervous system -neurospheres show increased expansion with increased G-CSF concentrations, in contrast to central nervous system - derived spheres. PMID: 24253464
- G-CSF STAT3 axis constitutes a key protective mechanism induced by injury to reduce the risk for posttraumatic infection. PMID: 24470495
- After deletion of Pten in mice lacking G-CSF, the splenomegaly, myeloproliferative disease, and splenic hematopoietic stem cell accumulation are rescued. PMID: 24127490
- Our results suggest 5-AED survival enhancement is G-CSF-dependent, and that it stimulates innate immune cell function and reduces radiation-induced DNA damage via induction of genes that modulate cell cycle progression and apoptosis. PMID: 22843381
- activation of the RAS/MEK/ERK pathway regulates G-CSF expression through the Ets transcription factor PMID: 23530240
- G-CSF protein is necessary and sufficient to restore monocyte chemoattractant protein (MCP)-1 deficiency in neutrophil-mediated host immunity following Klebsiella pneumoniae lung infection. PMID: 23129755
- Ang2 deficiency results in enhanced recruitment of myeloid cells depending on G-CSF, and that the enhancement results in more aggressive tumor growth and neo-angiogenesis during liver colonization. PMID: 22699974
- Il-1rn knockout mice exhibit delayed resolution in acute lung inflammation after exposure to lipopolysaccharide, a process that appears to be mediated via the granulocyte colony-stimulating factor/IL-17A axis. PMID: 22592923
- Induction of Bv8 expression by granulocyte colony-stimulating factor in CD11b+Gr1+ cells: key role of Stat3 signaling. PMID: 22528488
- BMMCs and G-CSF co-administration exhibits synergistic beneficial effect over time. PMID: 21699735
- granulocyte and macrophage populations of murine bone marrow cells are regulated by G-CSF and CD137 protein PMID: 21179444
- TLR2 signaling in G-CSF mobilized PBSCs correlates with their ability to rapidly differentiate into myeloid cells, resulting in improved engraftment PMID: 21239180
- G-CSF is crucial for skeletal myocyte development and regeneration PMID: 21422169
- G-CSF and IL-6 provide signals that determine the angiogenic potential of BM resident monocytes. PMID: 20354107
- G-CSF stimulates the expression of the MIP-2 receptor via STAT3-dependent transcriptional activation of Il8rb PMID: 20185584
- G-CSF exerts potent anti-apoptotic activities towards motoneurons in vivo and suggests that the protection offered by G-CSF in ALS mouse models is due to its direct neuroprotective activity PMID: 20178614
- Overexpression of STAT3beta did not alter the kinetics of G-CSF-mediated neutrophilic differentiation or p27 induction in 32D/G-CSF-R WT cells. PMID: 11920194
- Increased production of G-CSF in mice mounting the acute phase response is a key physiological component of host defense. PMID: 12097396
- Evaluation of role of G-CSF in the production, survival, and release of neutrophils from bone marrow into circulation using G-CSF-deficient mice PMID: 12130495
- G-CSF induces neutrophil mobilization indirectly through the generation of trans-acting signals PMID: 12387736
- G-CSF mediates granulopoiesis and, as a corollary, participates in neutrophilia in LFA-1 deficient mice. PMID: 12734371
- regulation of G-CSF levels may provide a mechanism for directing primitive hematopoietic progenitors into the common myeloid lineage in response to environmental stresses PMID: 12893769
- G-CSF treatment leads to Lyn-mediated tyrosine phosphorylation of Gab2, which may serve as an important intermediate of enhanced Akt activity and myeloid differentiation, not growth/survival response. PMID: 14656892
- G-CSF signaling in neutrophils is negatively regulated by SOCS3 PMID: 14699146
- observations show that lipocalin 24p3 is not involved in the granulocyte-colony stimulating factor withdrawal-induced apoptosis PMID: 14703690
- critical role for G-CSF in driving joint inflammation and as a potential therapeutic target in inflammatory joint diseases, such as rheumatoid arthritis. PMID: 15272075
- G-CSF induces stabilization of Fli-1 protein in myeloid cells PMID: 15557108
- G-CSF treatment significantly improved survival and liver histology in chemically injured mice, predominantly by promoting endogenous repair mechanisms; immunohistochemistry showed a higher percentage of bone marrow-origin hepatocytes PMID: 15661404
- Febrile-range hyperthermia-induced expression of G-CSF drives the sustained peripheral neutrophilia that occurs during sustained (36 h) hyperthermia in a conscious temperature-clamped mouse model. PMID: 15829718
- G-CSF modulates angiogenesis by increasing myelomonocytic cells (VEGFR1+ neutrophils) and their release of VEGF PMID: 16223785
- monocytes mobilized into the blood by G-CSF or AMD3100 stimulate angiogenesis at sites of ischemia through a paracrine mechanism PMID: 16735597
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亞細(xì)胞定位:Secreted.
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蛋白家族:IL-6 superfamily
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