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  4. Mouse Epidermal growth factor-like protein 6(EGFL6) ELISA kit

Mouse Epidermal growth factor-like protein 6(EGFL6) ELISA kit

  • 中文名稱：
    小鼠表皮生長(zhǎng)因子樣蛋白6(EGFL6)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL007475MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠表皮生長(zhǎng)因子樣蛋白6(EGFL6)酶聯(lián)免疫試劑盒（CSB-EL007475MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的EGFL6含量。EGFL6是一種重要靶點(diǎn)。它在細(xì)胞增殖、遷移等生理過(guò)程中發(fā)揮作用。研究發(fā)現(xiàn)其異常表達(dá)與多種疾病相關(guān)，如腫瘤的發(fā)生發(fā)展?？蒲腥藛T正深入探究其作用機(jī)制，期望通過(guò)靶向EGFL6開發(fā)新的診斷方法和治療策略。試劑盒檢測(cè)范圍為93.75 pg/mL-6000 pg/mL，科研人員可通過(guò)探究EGFL6在小鼠模型中的功能特性，例如在腫瘤發(fā)生、血管異常增生或創(chuàng)傷修復(fù)等研究領(lǐng)域，定量分析不同實(shí)驗(yàn)條件下EGFL6的動(dòng)態(tài)變化，為疾病機(jī)制研究或藥物干預(yù)效果評(píng)估提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Egfl6 ELISA Kit; Maeg ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; Protein W80 ELISA Kit
  • 縮寫：
    EGFL6
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    93.75 pg/mL-6000 pg/mL
  • 靈敏度：
    23.43 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-94
    1:2Average %98
    Range %94-101
    1:4Average %102
    Range %94-108
    1:8Average %94
    Range %87-97
  • 回收率：
    The recovery of mouse EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9794-103
    EDTA plasma (n=4)9389-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    60002.414 2.355 2.385 2.297
    30001.612 1.564 1.588 1.500
    15001.052 1.090 1.071 0.983
    7500.688 0.633 0.661 0.573
    3750.459 0.414 0.437 0.349
    187.50.298 0.280 0.289 0.201
    93.750.175 0.165 0.170 0.082
    00.088 0.087 0.088
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly.
  • 基因功能參考文獻(xiàn)：
    1. EGLF6 mediates a paracrine mechanism of cross-talk between vascular endothelial cells and osteoblasts PMID: 21531721
    2. MAEG may play a role as a mediator regulating epithelial-mesenchymal interaction through binding to RGD-binding integrins including alpha8beta1 during hair follicle development. PMID: 15572035
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix, basement membrane.
  • 蛋白家族：
    Nephronectin family
  • 組織特異性：
    Expressed at basement membrane of pelage follicles (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:54156

    UniGene: Mm.37707