亚洲一区二区三区中文字幂,在线观看免费人成视频色9,最近最新高清中文字幕,阳茎伸入阳道全过程视频,中出老熟女中文字幕

Your Good Partner in Biology Research

Mouse 5-Hydroxytryptamine,5HT ELISA Kit

  • 中文名稱:
    小鼠5羥色胺(5-HT)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08365m
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠5羥色胺(5-HT)酶聯(lián)免疫試劑盒(CSB-E08365m)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的5HT含量。5-HT即血清素,是一種重要的神經遞質。它廣泛分布于中樞神經系統(tǒng)與外周組織。在研究機制上,參與調節(jié)情緒、睡眠、食欲等多種生理功能,異常水平與抑郁癥等精神疾病相關。當前圍繞其開展的研究,旨在開發(fā)相關藥物來治療各類身心疾病。試劑盒檢測范圍為250 pg/mL-4000 pg/ml,該產品適用于神經科學研究中的遞質動態(tài)分析、藥理學實驗的干預效果評估、行為學模型驗證以及代謝相關疾病動物模型的機制探索本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    5HT
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    250 pg/mL-4000 pg/mL
  • 靈敏度:
    125 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 90-89  
    1:200 Average % 90  
    Range % 86-94  
    1:400 Average % 84  
    Range % 80-88  
    1:800 Average % 97  
    Range % 92-101  
  • 回收率:
    The recovery of mouse 5HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-99  
    EDTA plasma (n=4) 89 83-96  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    4000 0.130 0.120 0.125    
    2000 0.243 0.241 0.242    
    1000 0.452 0.419 0.436    
    500 0.792 0.831 0.812    
    250 1.068 1.120 1.094    
    0 2.151 2.026 2.089    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

平均分:
4.5分 - 2 個評價

樣品類型:細胞培養(yǎng)上清液

樣品信息:小鼠

稀釋比:其他 1:2

產品評價: 我用檢測PC12細胞上清液,標準曲線的OD值為y = -1.0317x + 2.6063,R2 = 0.9866 樣本的OD值為0.1084~0.2402 快速便捷,非常好用

By 廖老師

樣品類型:其他樣品處理方法 細胞勻漿液,用適量ripa裂解后取離心后上清做

樣品信息:小鼠

稀釋比:其他 1:20

產品評價: CSB-E08365m檢測不同濃度的戊酸給給藥后的LLC培養(yǎng)上清液以及細胞勻漿液的5HT含量,標準曲線的OD值為:4000-0.08,2000-0.15,1000-0.17,500-0.43,250-0.87,0-1.01,樣品OD值為0.19~0.67,陰性對照是0.99左右,產品快速便捷,操作簡單,重復性較好,但是貌似沒有對細胞樣品的處理指南。陰性對照是水(0.9900,0.9142,0.9521),樣品則為0.19~0.67;標曲的0濃度吸光值為(1.0943,1.0101)。

By 廖老師