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  4. Bovine Interleukin 12,IL-12 ELISA Kit

Bovine Interleukin 12,IL-12 ELISA Kit

  • 中文名稱：
    牛白介素12(IL-12)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14010B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛白介素12(IL-12)酶聯(lián)免疫試劑盒（CSB-E14010B）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL12A含量。IL12A為重要靶點(diǎn)，在免疫系統(tǒng)中扮演關(guān)鍵角色。其背景上，IL-12由IL12A等亞基組成，可調(diào)節(jié)免疫反應(yīng)。研究機(jī)制方面，它能促進(jìn)T細(xì)胞和NK細(xì)胞的活化、增殖，增強(qiáng)免疫細(xì)胞對(duì)病原體和腫瘤細(xì)胞的殺傷作用，是免疫治療的研究熱點(diǎn)。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品為研究牛類感染性疾病、炎癥反應(yīng)機(jī)制及疫苗免疫效果評(píng)估提供可靠工具，尤其適用于畜牧科研中免疫調(diào)節(jié)機(jī)制探索、疾病模型建立等基礎(chǔ)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %98
    Range %91-102
    1:4Average %94
    Range %90-98
    1:8Average %94
    Range %86-99
  • 回收率：
    The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-92
    EDTA plasma (n=4)9795-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.758 2.628 2.693 2.531
    2002.184 2.084 2.134 1.972
    1001.612 1.562 1.587 1.425
    501.203 1.173 1.188 1.026
    250.652 0.642 0.647 0.485
    12.50.384 0.374 0.379 0.217
    6.250.258 0.253 0.256 0.094
    00.163 0.161 0.162 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 基因功能參考文獻(xiàn)：
    1. The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193
    2. Lipopolysaccharide stimulation up-regulates the secretion of cytokines, including interleukin-12, by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: bta:281856

    STRING: 9913.ENSBTAP00000020153

    UniGene: Bt.524