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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Bovine Epithelial growth factor(EGF)ELISA Kit

Bovine Epithelial growth factor(EGF)ELISA Kit

  • 中文名稱：
    牛表皮生長(zhǎng)因子(EGF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17171B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛表皮生長(zhǎng)因子(EGF)酶聯(lián)免疫試劑盒（CSB-E17171B）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的EGF含量。EGF（表皮生長(zhǎng)因子）是EGF家族的創(chuàng)始成員，具有53個(gè)氨基酸殘基和三個(gè)分子內(nèi)二硫鍵。通過(guò)與細(xì)胞表面的EGFR受體結(jié)合，EGF刺激細(xì)胞生長(zhǎng)、分化和存活，參與多種生理和病理過(guò)程，如組織再生、傷口愈合和腫瘤發(fā)生。EGF通過(guò)激活EGFR的酪氨酸激酶活性，啟動(dòng)信號(hào)轉(zhuǎn)導(dǎo)級(jí)聯(lián)反應(yīng)，導(dǎo)致細(xì)胞內(nèi)鈣水平升高、糖酵解和蛋白質(zhì)合成增加，最終促進(jìn)DNA合成和細(xì)胞增殖。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品可廣泛應(yīng)用于動(dòng)物生理學(xué)研究、營(yíng)養(yǎng)代謝評(píng)估、疾病模型構(gòu)建等科研領(lǐng)域，為探究牛EGF在生長(zhǎng)發(fā)育調(diào)控、免疫應(yīng)答及病理過(guò)程中的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    HOMG4, URG, beta-urogastrone|epidermal growth factor
  • 縮寫：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %88-97
    1:2Average %106
    Range %100-109
    1:4Average %90
    Range %85-94
    1:8Average %94
    Range %90-97
  • 回收率：
    The recovery of bovine EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-97
    EDTA plasma (n=4)9489-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.109 2.042 2.076 1.952
    51.710 1.723 1.717 1.593
    2.51.432 1.421 1.427 1.303
    1.251.018 0.999 1.009 0.885
    0.6250.678 0.668 0.673 0.549
    0.3120.466 0.451 0.459 0.335
    0.1560.299 0.292 0.296 0.172
    00.125 0.123 0.124
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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