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  4. Bovine Apolipoprotein A-I(APOA1) ELISA kit

Bovine Apolipoprotein A-I(APOA1) ELISA kit

  • 中文名稱：
    牛載脂蛋白A1(APOA1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL001913BO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛載脂蛋白A1(APOA1)酶聯(lián)免疫試劑盒（CSB-EL001913BO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是重要的載脂蛋白，在脂質(zhì)代謝中意義重大。研究顯示，其能促進(jìn)膽固醇逆向轉(zhuǎn)運(yùn)，抑制動(dòng)脈粥樣硬化。還可穩(wěn)定高密度脂蛋白結(jié)構(gòu)，發(fā)揮抗炎癥、抗氧化作用。對(duì)其深入研究有望為心血管疾病等的防治提供新思路。試劑盒檢測(cè)范圍為78 ng/mL-5000 ng/mL，本試劑盒適用于探索牛脂質(zhì)代謝相關(guān)疾病機(jī)制、評(píng)估營(yíng)養(yǎng)干預(yù)對(duì)脂蛋白合成的影響，或作為動(dòng)物模型中動(dòng)脈粥樣硬化研究的工具，為畜牧養(yǎng)殖健康管理、動(dòng)物疾病模型構(gòu)建及基礎(chǔ)醫(yī)學(xué)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    78 ng/mL-5000 ng/mL
  • 靈敏度：
    19.5 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 90  
    Range % 87-94  
    1:2000 Average % 96  
    Range % 91-100  
    1:4000 Average % 93  
    Range % 87-97  
    1:8000 Average % 100  
    Range % 96-103  
  • 回收率：
    The recovery of bovine APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 82-87  
    EDTA plasma (n=4) 89 85-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    5000 0.140 0.143 0.142    
    2500 0.285 0.291 0.288    
    1250 0.482 0.503 0.493    
    625 0.814 0.821 0.818    
    312 1.253 1.268 1.261    
    156 1.695 1.709 1.702    
    78 2.040 2.134 2.087    
    0 2.436 2.458 2.447    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 基因功能參考文獻(xiàn)：
    1. the model of a two-step process for the transendothelial transport of apoA-I in which apoA-I is initially lipidated by ABCA1 and then further processed by ABCA1-independent mechanisms. PMID: 21209084
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 組織特異性：
    Major protein of plasma HDL, also found in chylomicrons.
  • 數(shù)據(jù)庫鏈接：